Tumor Necrosis Factor Alpha (TNFα) ELISA

BioMarkers | Diabetic Nephropathy | Inflammation


The K-ASSAY®Human Tumor Necrosis Factor alpha (TNFα) ELISA is an enzyme-linked immunosorbent assay for the quantitative determination of TNFα in human cell culture supernatant, serum and plasma.

TNFα is a central regulator of inflammation and represents one of the cytokines of the acute phase response in the liver. It is produced by a variety of cell types, such as macrophages, lymphocytes and endothelial cells. Large amounts of TNF are released following exposure to bacterial products (for example lipopolysaccharide) as well as interleukins (IL)-1 and -6.

Upregulation of TNFα is observed in several inflammatory diseases including: inflammatory bowel disease (IBD), psoriasis, rheumatoid arthritis, diabetic nephropathy and cardiovascular diseases. Purified TNFα has shown cytotoxic and cytostatic activities against various human tumor cell lines.



Species reactivity


Sample type

Cell culture supernatant, serum, plasma

Assay type

Sandwich (quantitative)


15.6–1,000 pg/mL

Detection method


Assay time

3h 30min


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Check also our collection of ANIMAL TNFα ELISA Kits


Partner: Kamiya BioMedical Company

TNFα ELISA - Product Insert | "Research Use Only"

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