Human Collagen III (COL3) ELISA Kit
Human K-ASSAY® Collagen III (COL3) ELISA Kit - For the quantitative determination of human Collagen III (COL3) in serum, plasma, tissue homogenates and other biological fluids.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL3. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain COL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 10 nm. The concentration of COL3 in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
Product Number: KT-11210
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Supplier: Kamiya Biomedical Company