Human Collagen IV ELISA (serum)

The Human K-ASSAY® Collagen IV (COL4) ELISA Kit is a proven quantitative assay for intact Collagen IV in human serum samples. 

Easy to Use: 

• Only 20 uL human serum required.
• Standard 96-well quantitative microtiter ELISA format.

Accurate: 

• Monoclonal antibodies detect only intact Collagen IV.
• Highly sensitive assay (15.6 - 1,000 ug/L measuring range).
• Excellent precision and reproducibility.
• No significant interference.
• No significant cross reactivity with other collagen isoforms.
• Early and sensitive biomarker of collagen deposition.

Clinical Research Areas: 

• Liver fibrosis 
• Chronic liver disease
• Viral hepatitis
• Hepatocellular carcinoma
• Alcoholism
• Hepatotoxicity
• Liver cirrhosis

Principle:

The K-ASSAY® Human Collagen IV ELISA Kit (serum) is a solid phase one-step sandwich ELISA. Collagen IV in the sample is bound simultaneously by a solid phase monoclonal antibody and a monoclonal antibody-enzyme conjugate, each directed at different antigenic sites. This results in the collagen IV molecule being sandwiched between the solid phase and enzyme-labelled antibodies. After removing unbound enzyme-labelled antibody and sample, the plate is incubated with enzyme substrate. The resultant color development is directly proportional to the amount of collagen IV in the sample. 

Product Number: KT-035

Working with urine samples? please check out the Collagen IV ELISA kit (urine)

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Supplier: Kamiya Biomedical Company