Vitamin C Assay (Chemistry)

The K-ASSAY® Vitamin C Assay kit for the quantitative determination of Vitamin C in serum, plasma, food, beverages, and other biological samples.

Cobo Scientific is pleased to announce the new K-ASSAY® Vitamin C Assay Reagent and Calibratorfrom Kamiya Biomedical Company.

The assay offers many advantages:

• Exclusive formula. U.S. patent # 6,153,399.
• Low price per test.
• Reliable performance.  
• Assay range from 0.5 to 50.0 ug/mL.
• Usable on serum, plasma, and other samples on most open channel chemistry analyzers.
• The included Sample Stabilizer increases sample usability for up to 2 weeks.

Introduction:

Vitamin C (L-Ascorbic acid) is a water-soluble vitamin with strong reduction ability and is important as a coenzyme of the internal hydroxylation reactions such as with collagen composition. Vitamin C is divided into reduced form (Ascorbic acid (AsA) and oxidized form (dehydroascorbic acid (DHAsA)). This vitamin C assay measures total vitamin C (AsA and DHAsA). Vitamin C is a six-carbon lactone that is synthesized from glucose in the liver of most mammalian species, but not by humans. Therefore, humans must obtain ascorbate in their diet in order to survive. In humans, ascorbate acts as an electron donor for eight different enzymes. It also serves as an antioxidant and may be beneficial for reducing the risk of developing chronic diseases such as cancer, cardiovascular disease, and cataracts. 

Principle:

The K-ASSAY® Vitamin C Assay quantifies the vitamin C in serum or plasma based on an enzymic method. Ascorbate Oxidase catalyzes the oxidation of L-Ascorbate (vitamin C) to generate hydrogen peroxide. Peroxidase catalyzes the hydrogen peroxide and the unique chromogen to form blue dye, which enables high sensitive measurement. The reduction effect of Vitamin C has no effect on this reagent. The K-ASSAY® Vitamin C assay can be run using a two reagent clinical chemistry analyzer. The K-ASSAY® Vitamin C Calibrator is used in conjunction with a 0-point saline to prepare a 2-point calibration. This calibration is then used for quantifying the levels of vitamin C present in serum or plasma samples.

Product Numbers: Reagent (KT-75000) / Calibrator (KT-75001)

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Supplier: Kamiya Biomedical Company