Combining Zebrafish and CRISPR/Cas9: Toward a More Efficient Drug Discovery Pipeline
"This review will focus on the most recent advances on CRISPR/Cas9 implementation in zebrafish and all their potential uses in biomedical research and drug discovery"
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Zebrafish Services - Functional Genomics and Target Validation
High-Throughput CRISPR-based Screening in Zebrafish
If you are involved in functional genomics or target validation and wish to benefit from recent advances in CRISPR technology without impacting your resources and timelines…
Why not outsourcing your gene editing work to a specialist team?
Our partner, ZeClinics, is the leading zebrafish company in Europe and experts in using CRISPR/Cas9* to do both simple and advanced genome editing in zebrafish.
- Single and double Knock-outs
- Conditional Knock-outs
- Short and large Knock-ins
- Targeted gene mutation
- Gene tagging
You can have your founder animals as soon as 3 months and can start screening for the relevant phenotype using one of the many phenotypic assays offered (see examples below).
Phenotypic Assays - Overview
Neuroprotection
- Locomotion characterisation
- Motoneuron cluster confocal imaging
Cardioprotection
- Heart beat frequency (Ventricle and Atrium)
- Arrhythmia
- QT prolongation (A/V ratio)
- Fibrillation
- Cardiac arrest
- Ejection Fraction
- Vein/Artery Blood Flow Velocity
Atherosclerosis
- Whole body measurement of cholesterol levels
- Image analysis of cholesterol vascular accumulation
Duchene Muscular Dystrophia
- Locomotion characterization
- Optical birefringence.
- Genotyping
- Immuno-histochemistry
Hearing Loss Assay
- Phenotypical saccular macula analysis
- Confocal microscopy functionality assessment
- Sound response assay
- Phenotypical scoring of mutants
Parkinson & Epilepsy behavioural assays
- Locomotion characterization
- Convulsion characterization
- Thigmotaxis evaluation
- Stimuli habituation (light/dark, sound and tapping responses)
- Neuronal morphology confocal imaging
Click on the link to find out more about our this powerful Genome Editing Service Platform in Zebrafish.
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*ZeClinics has the CRISPR/Cas9 license from the Broad Institute, MIT, Harvard University
