Detection of Large Deletions in CRISPR Experiments

It can be challenging to detect large deletions in CRISPR experiments using the standard Sanger/NGS approach.

Here we show the power of the INDEL Profiling Platform, to easily detect a large deletion (app. 600bs) in a pool of human cells and confirm 100 % bi-allelic deletion in a selected clone.

Customer did targeted deletion using 2 gRNAs, extracted DNA from cells and shipped the genomic DNA to COBO Technologies. COBO did tri-primer PCR, INDEL analysis and bioinformatic using ProfileIt and reported back results within only 5 days

The data was generated in collaboration with a research group at the Karolinska Institute in Sweden.

Detection of Large Deletions in CRISPR Experiments
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